Learning Epigenetics from ShirleyLiu

1 Transcription Regulation & motif finding from: https://www.youtube.com/watch?v=K2gKdFPipv0&feature=youtu.be&ab_channel=XiaoleShirleyLiu TFs are proteins combine specific sequence and they usually recognize a sequnece motif or a pattern, which will regulate the nearby gene expression. 2 Epigenetics from: https://www.

Things about High-throughput Sequencing

Complementary approaches Mainly four steps of illumina sequencing library construction cluster formation sequencing data analysis Library prep RNA-seq? Actually DNA sequencing We cannot directly sequencing the RNA. We can only reverse transcript the RNA(AUCG) to DNA (ATCG) then do sequencing.

Basic Cancer Biology

Coursera~Johns Hopkins I recently learnt some basic cancer biology from coursera, the link as below. https://www.coursera.org/learn/cancer/lecture/XsshR/incidence-and-etiology-of-cancer Lecture1~ Cancer Terms and Definitions Cancer means “uncontrolled growth” => uncontrolled devision of abnormal cells in a part of the body

Knowledge about BRCA

Read content on this web: https://www.cancer.gov/about-cancer/causes-prevention/genetics/brca-fact-sheet https://www.cancer.gov/about-cancer/treatment/types/precision-medicine/tumor-dna-sequencing What are BRCA1 and BRCA2? BRCA1 and BRCA2 are human genes that produce tumor suppressor proteins. Specific inherited mutations in BRCA1 and BRCA2 most notably increase the risk of female breast and ovarian cancers.

Introduction to mRNA Microarray

About terminology target: DNA hybridized to the array, mobile substrate probe: DNA spotted on the array (spot) ; one of these 25-mer oligonucleutides probe set: a collection of probes (e.g. 11) targeting the same transcript print-tip-group: collection of spots printed using the same print-tip (or pin), aka.