I graduated from Shandong Agricultural University. After one year study, with the help of Prof. Aijun QU and Prof. Fangqiang ZHENG, I got a chance for 2-year joint education at the Institute of Plant Protection, Chinese Academy of Agricultural Science. At first, my job was doing some basic ecological and molecular experiments (e.g. raising cotton bollworms to detect Cry1Ac toxin resistance; DNA/RNA exaction; RT-PCR). Until December 2017, I received a 15-sample RNA-Seq analysis project from my senior fellow and then, I began self-learning on RNA-Seq analysis (without reference genome).
It is never a smooth ride. As the only bioinformatics learner in my lab, I have no programming background, no concept about NGS, and no computing resources etc. But what I have is patience and perseverance. I started from learning NGS sequencing development and knew how the raw data was produced. I read R and Shell tutorials to learn how to write codes. Thanks to Bioconductor for providing so much helpful vignettes about RNA-Seq downstream analysis and visualization. At last, the analysis were completed in 40 days and I made my first heatmap just using codes (still remember the exciting moment) !
From then on, I found the love of diving into bioinformatics filed. In such a moment, I felt that I was a magician and could help others to turn fussy file into fancy figure. On June 21, 2019, I graduated with master degree with a dissertation on Helicoverpa armigera RNA-Seq analysis.
From June to December in 2019, I worked in Jimmy’s company (JM Bio) in Zhuhai, of course, with my girlfriend Xiaojie (she is the R programming lecturer). I focused on single-cell analysis and keep track of the development in this cutting-edge filed.
Now, I am a research assistant in University of Macau focusing on epigenomics (ChIP-Seq + RNA-Seq) meanwhile pursuing a doctorate. I gained experience in handling the RNA-Seq (with or without reference genome), ChIP-Seq, scRNA analysis and public database use like GEO, TCGA. Also I have a great interest in WES, microarray, ATAC-Seq. In the future, I would like to integrate my scripts into much standard pilelines in my github.
According to my own learning curve, I care much about open bioinformatics communication and sharing community. I frequently share my ideas, tips and knowledge in my Jianshu Web (started from 2018.6.5). For now, I have written 350+ articles over 700,000+ words. But unfortunately, due to the unproper management of Jianshu, I have to give up this platform with mixed feelings.
To record our learning progress, my girlfriend (Xiao Jie) and I (Yunze) started the Wechat Official Account “ 生信星球"(Bioinfoplanet) on May 11, 2018 and that is why my website’s domain is called “jieandze1314”. Now, she is my wife. Hand in hand , till grow old with you.
We insist on publishing nearly every day to our 18000+ followers and know how hard to step into the bioinformatics palace. To better spread the bioinformatics skills, we have hosted 100+ online trainings for 700+ newbs and met lots of hard-working and lovely trainees. Their favourable comments also make us feel more resonsibility and happiness.( comment1, comment2, comment3)